ARHGEF6 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ARHGEF6 transfected lysate.
Western Blot
Lane 1: ARHGEF6 transfected lysate ( 85.47 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ARHGEF6 full-length
Host
Human
Theoretical MW (kDa)
85.47
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ARHGEF6 antibody (H00009459-B01) by Western Blots.
SDS-PAGE Gel
ARHGEF6 transfected lysate.
Western Blot
Lane 1: ARHGEF6 transfected lysate ( 85.47 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ARHGEF6
Entrez GeneID
9459GeneBank Accession#
NM_004840Protein Accession#
NP_004831Gene Name
ARHGEF6
Gene Alias
COOL2, Cool-2, KIAA0006, MRX46, PIXA, alpha-PIX, alphaPIX
Gene Description
Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. [provided by RefSeq
Other Designations
OTTHUMP00000062283|PAK-interacting exchange factor, alpha|Rac/Cdc42 guanine exchange factor (GEF) 6|Rac/Cdc42 guanine nucleotide exchange factor 6|mental retardation, X-linked 46|rho guanine nucleotide exchange factor 6
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Interactome
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Pathway
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Disease
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