ARHGEF6 monoclonal antibody (M02), clone 2E5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody (M02), clone 2E5.
Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of ARHGEF6 transfected lysate using anti-ARHGEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF6 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARHGEF6 is approximately 0.03ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CDC42 and ARHGEF6. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-ARHGEF6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot detection against Immunogen (38.06 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ARHGEF6.
Immunogen
ARHGEF6 (AAH39856.1, 61 a.a. ~ 175 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QTEADCINNINDFLKGCATLQVEIFDPDDLYSGVNFSKVLSTLLAVNKATEDQLSERPCGRSSSLSAANTSQTNPQGAVSSTVSGLQRQSKTVEMTENGSHQLIVKARFNFKQTN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38.06 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody (M02), clone 2E5.
Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of ARHGEF6 transfected lysate using anti-ARHGEF6 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF6 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARHGEF6 is approximately 0.03ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CDC42 and ARHGEF6. HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-ARHGEF6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — ARHGEF6
Entrez GeneID
9459GeneBank Accession#
BC039856Protein Accession#
AAH39856.1Gene Name
ARHGEF6
Gene Alias
COOL2, Cool-2, KIAA0006, MRX46, PIXA, alpha-PIX, alphaPIX
Gene Description
Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. [provided by RefSeq
Other Designations
OTTHUMP00000062283|PAK-interacting exchange factor, alpha|Rac/Cdc42 guanine exchange factor (GEF) 6|Rac/Cdc42 guanine nucleotide exchange factor 6|mental retardation, X-linked 46|rho guanine nucleotide exchange factor 6
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Interactome
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Pathway
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Disease
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Publication Reference
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ARF1 recruits RAC1 to leading edge in neutrophil chemotaxis.
Mazaki Y, Onodera Y, Higashi T, Horinouchi T, Oikawa T, Sabe H.
Cell Communication and Signaling 2017 Oct; 15(1):36.
Application：IF, WB-Tr, Human , HL-60 cells.
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ARF1 recruits RAC1 to leading edge in neutrophil chemotaxis.
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