FHL5 monoclonal antibody (M01), clone 1G12-D2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant FHL5.
Immunogen
FHL5 (AAH21723, 1 a.a. ~ 284 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTTAHFYCQYCTASLLGKKYVLKDDSPYCVTCYDRVFSNYCEECKKPIESDSKDLCYKDRHWHEGCFKCTKCNHSLVEKPFAAKDERLLCTECYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCENCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFCDQLWHKECFLCSDCRKDLCEEQFMSRDDYPFCMDCYNHLYANKCVACSKPISGLTGAKFICFQDSQWHSECFNCGKCSVSLVGKGFLTQNKEIFCQKCGSGMDTDI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (86)
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (56.98 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of FHL5 expression in transfected 293T cell line by FHL5 monoclonal antibody (M01), clone 1G12-D2.
Lane 1: FHL5 transfected lysate(32.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to FHL5 on HepG2 cell. [antibody concentration 10 ug/ml] -
Gene Info — FHL5
Entrez GeneID
9457GeneBank Accession#
BC021723Protein Accession#
AAH21723Gene Name
FHL5
Gene Alias
ACT, FLJ33049, KIAA0776, RP3-393D12.2, dJ393D12.2
Gene Description
four and a half LIM domains 5
Omim ID
605126Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. [provided by RefSeq
Other Designations
1700027G07Rik|LIM protein ACT|OTTHUMP00000016879|activator of cAMP-responsive element modulator (CREM) in testis
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Interactome
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Disease
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