CRSP9 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MED7 transfected lysate.
Western Blot
Lane 1: MED7 transfected lysate ( 25.74 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CRSP9 full-length
Host
Human
Theoretical MW (kDa)
25.74
Interspecies Antigen Sequence
Mouse (96); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CRSP9 antibody (H00009443-B01) by Western Blots.
SDS-PAGE Gel
MED7 transfected lysate.
Western Blot
Lane 1: MED7 transfected lysate ( 25.74 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MED7
Entrez GeneID
9443GeneBank Accession#
NM_004270.3Protein Accession#
NP_004261.1Gene Name
MED7
Gene Alias
CRSP33, CRSP9, MGC12284
Gene Description
mediator complex subunit 7
Omim ID
605045Gene Ontology
HyperlinkGene Summary
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000160735|cofactor required for Sp1 transcriptional activation, subunit 9 (33kD)|cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
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Interactome
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Disease
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