Products

Western Blot (Cell lysate)

CRSP9 monoclonal antibody (M01), clone 3E7 Western Blot analysis of CRSP9 expression in Jurkat ( Cat # L017V1 ).

Western Blot (Transfected lysate)

Western Blot analysis of CRSP9 expression in transfected 293T cell line by CRSP9 monoclonal antibody (M01), clone 3E7.

Lane 1: CRSP9 transfected lysate(27.2 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged CRSP9 is approximately 0.3ng/ml as a capture antibody.

ELISA

RNAi Knockdown (Antibody validated)

Western blot analysis of CRSP9 over-expressed 293 cell line, cotransfected with CRSP9 Validated Chimera RNAi ( Cat # H00009443-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRSP9 monoclonal antibody (M01), clone 3E7 (Cat # H00009443-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Entrez GeneID

9443

GeneBank Accession#

BC005250

Protein Accession#

AAH05250

Gene Name

MED7

Gene Alias

CRSP33, CRSP9, MGC12284

Gene Description

mediator complex subunit 7

Omim ID

605045

Gene Ontology

Hyperlink

Gene Summary

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq

Other Designations

OTTHUMP00000160735|cofactor required for Sp1 transcriptional activation, subunit 9 (33kD)|cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa