CRSP8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
MED27 transfected lysate.
Western Blot
Lane 1: MED27 transfected lysate ( 34.32 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CRSP8 full-length
Host
Human
Theoretical MW (kDa)
34.32
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CRSP8 antibody (H00009442-B01) by Western Blots.
SDS-PAGE Gel
MED27 transfected lysate.
Western Blot
Lane 1: MED27 transfected lysate ( 34.32 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MED27
Entrez GeneID
9442GeneBank Accession#
NM_004269.2Protein Accession#
NP_004260.2Gene Name
MED27
Gene Alias
CRAP34, CRSP34, CRSP8, MGC11274, TRAP37
Gene Description
mediator complex subunit 27
Omim ID
605044Gene Ontology
HyperlinkGene Summary
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq
Other Designations
cofactor required for Sp1 transcriptional activation, subunit 8 (34kD)|cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa|p37 TRAP/SMCC/PC2 subunit
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