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CRSP8 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00009442-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CRSP8 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 34.32
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CRSP8 antibody (H00009442-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00009442-T01
    MED27 transfected lysate.
    Western Blot
    QC Testing of H00009442-T01
    Lane 1: MED27 transfected lysate ( 34.32 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 9442
  • Gene Name:
  • MED27
  • Gene Alias:
  • CRAP34,CRSP34,CRSP8,MGC11274,TRAP37
  • Gene Description:
  • mediator complex subunit 27
  • Gene Summary:
  • The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq
  • Other Designations:
  • cofactor required for Sp1 transcriptional activation, subunit 8 (34kD),cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa,p37 TRAP/SMCC/PC2 subunit
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