CRSP7 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CRSP7 full-length
Host
Human
Theoretical MW (kDa)
65.4
Interspecies Antigen Sequence
Mouse (84); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DFFA antibody (H00009441-D01P) by Western Blots.
SDS-PAGE Gel
MED26 transfected lysate.
Western Blot
Lane 1: MED26 transfected lysate ( 65.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MED26
Entrez GeneID
9441GeneBank Accession#
NM_004831.3Protein Accession#
NP_004822.2Gene Name
MED26
Gene Alias
CRSP7, CRSP70
Gene Description
mediator complex subunit 26
Omim ID
605043Gene Ontology
HyperlinkGene Summary
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq
Other Designations
cofactor required for Sp1 transcriptional activation, subunit 7 (70kD)|cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa
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