CYP7B1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CYP7B1 transfected lysate.
Western Blot
Lane 1: CYP7B1 transfected lysate ( 58.3 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SPEX2 full-length
Host
Human
Theoretical MW (kDa)
58.3
Interspecies Antigen Sequence
Mouse (67); Rat (66)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CYP7B1 antibody (H00009420-B01) by Western Blots.
SDS-PAGE Gel
CYP7B1 transfected lysate.
Western Blot
Lane 1: CYP7B1 transfected lysate ( 58.3 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYP7B1
Entrez GeneID
9420GeneBank Accession#
NM_004820.2Protein Accession#
NP_004811.1Gene Name
CYP7B1
Gene Alias
CBAS3, CP7B, SPG5A
Gene Description
cytochrome P450, family 7, subfamily B, polypeptide 1
Omim ID
603711Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. [provided by RefSeq
Other Designations
cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1|oxysterol 7alpha-hydroxylase
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Interactome
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Pathway
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Disease
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