COX5A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-COX5A full-length
Host
Human
Theoretical MW (kDa)
16.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COX5A antibody (H00009377-D01P) by Western Blots.
SDS-PAGE Gel
COX5A transfected lysate.
Western Blot
Lane 1: COX5A transfected lysate ( 16.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — COX5A
Entrez GeneID
9377GeneBank Accession#
BC024240.2Protein Accession#
AAH24240.1Gene Name
COX5A
Gene Alias
COX, COX-VA, VA
Gene Description
cytochrome c oxidase subunit Va
Omim ID
603773Gene Ontology
HyperlinkGene Summary
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq
Other Designations
cytochrome c oxidase polypeptide, mitochondrial|mitochondrial cytochrome c oxidase subunit Va
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