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Last updated: 2017/6/25
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STK17A monoclonal antibody (M03), clone 4D12

  • Catalog # : H00009263-M03
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant STK17A.
  • Immunogen:
  • STK17A (AAH47696, 301 a.a. ~ 413 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • LLVKKPEDRATAEECLKHPWLTQSSIQEPSFRMEKALEEANALQEGHSVPEINSDTDKSETEESIVTEELIVVTSYTLGQCRQSEKEKMEQKAISKRFKFEEPLLQEIPGEFI
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00009263-M03
    Western Blot detection against Immunogen (38.54 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • STK17A monoclonal antibody (M03), clone 4D12 Western Blot analysis of STK17A expression in HepG2 ( Cat # L019V1 ).
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of STK17A expression in transfected 293T cell line by STK17A monoclonal antibody (M03), clone 4D12.

    Lane 1: STK17A transfected lysate(46.6 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) enlargeenlarge this image
  • Immunoperoxidase of monoclonal antibody to STK17A on formalin-fixed paraffin-embedded human colon cancer. [antibody concentration 1.2 ug/ml]
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  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged STK17A is approximately 0.1ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of STK17A over-expressed 293 cell line, cotransfected with STK17A Validated Chimera RNAi ( Cat # H00009263-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK17A monoclonal antibody (M03) clone 4D12 (Cat # H00009263-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9263
  • Gene Name:
  • STK17A
  • Gene Alias:
  • DRAK1
  • Gene Description:
  • serine/threonine kinase 17a
  • Gene Summary:
  • This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. [provided by RefSeq
  • Other Designations:
  • DAP kinase-related apoptosis-inducing protein kinase 1,death-associated protein kinase-related 1,serine/threonine kinase 17a (apoptosis-inducing)
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