PDLIM7 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PDLIM7 transfected lysate.
Western Blot
Lane 1: PDLIM7 transfected lysate ( 49.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PDLIM7 full-length
Host
Human
Theoretical MW (kDa)
49.8
Interspecies Antigen Sequence
Mouse (93); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PDLIM7 antibody (H00009260-D01) by Western Blots.
SDS-PAGE Gel
PDLIM7 transfected lysate.
Western Blot
Lane 1: PDLIM7 transfected lysate ( 49.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PDLIM7
Entrez GeneID
9260GeneBank Accession#
NM_005451.3Protein Accession#
NP_005442.2Gene Name
PDLIM7
Gene Alias
LMP1
Gene Description
PDZ and LIM domain 7 (enigma)
Omim ID
605903Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq
Other Designations
1110003B01Rik|LIM domain protein|PDZ and LIM domain 7
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