RPS6KA5 monoclonal antibody (M01), clone 2B11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RPS6KA5.
Immunogen
RPS6KA5 (AAH17187.1, 271 a.a. ~ 370 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ILKSEPPYPQEMSALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFFQKINWDDLAAKKVPAPFKPVIRDELDVSNFAEEFTEMDPTYSPAALPQSSEK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96)
Isotype
IgG2b Lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RPS6KA5 expression in transfected 293T cell line by RPS6KA5 monoclonal antibody (M01), clone 2B11.
Lane 1: RPS6KA5 transfected lysate(89.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between NR4A1 and RPS6KA5. HeLa cells were stained with anti-NR4A1 rabbit purified polyclonal 1:1200 and anti-RPS6KA5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to RPS6KA5 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RPS6KA5
Entrez GeneID
9252GeneBank Accession#
BC017187Protein Accession#
AAH17187.1Gene Name
RPS6KA5
Gene Alias
MGC1911, MSK1, MSPK1, RLPK
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 5
Omim ID
603607Gene Ontology
HyperlinkGene Summary
90kDa
Other Designations
mitogen- and stress-activated protein kinase 1|ribosomal protein S6 kinase, 90kD, polypeptide 5
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Interactome
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Pathway
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Disease
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