NOLC1 monoclonal antibody (M01), clone 3F8
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NOLC1.
Immunogen
NOLC1 (NP_004732, 590 a.a. ~ 699 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (72)
Isotype
IgG3 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NOLC1 monoclonal antibody (M01), clone 3F8. Western Blot analysis of NOLC1 expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of NOLC1 expression in transfected 293T cell line by NOLC1 monoclonal antibody (M01), clone 3F8.
Lane 1: NOLC1 transfected lysate (Predicted MW: 73.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NOLC1 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — NOLC1
Entrez GeneID
9221GeneBank Accession#
NM_004741Protein Accession#
NP_004732Gene Name
NOLC1
Gene Alias
KIAA0035, NOPP130, NOPP140, NS5ATP13, P130
Gene Description
nucleolar and coiled-body phosphoprotein 1
Omim ID
602394Gene Ontology
HyperlinkOther Designations
HCV NS5A trans-regulated protein 13|OTTHUMP00000020356|nucleolar and coiled-body phosphprotein 1|nucleolar phosphoprotein p130|nucleolar protein p130
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Interactome
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Disease
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