Western blot analysis of AURKB over-expressed 293 cell line, cotransfected with AURKB Validated Chimera RNAi ( Cat # H00009212-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AURKB monoclonal antibody (M04) clone 6G8 (Cat # H00009212-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM