LGI1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
LGI1 transfected lysate.
Western Blot
Lane 1: LGI1 transfected lysate ( 61.38 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LGI1 full-length
Host
Human
Theoretical MW (kDa)
61.38
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LGI1 antibody (H00009211-B01) by Western Blots.
SDS-PAGE Gel
LGI1 transfected lysate.
Western Blot
Lane 1: LGI1 transfected lysate ( 61.38 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LGI1
Entrez GeneID
9211GeneBank Accession#
NM_005097.1Protein Accession#
NP_005088.1Gene Name
LGI1
Gene Alias
EPITEMPIN, EPT, ETL1, IB1099
Gene Description
leucine-rich, glioma inactivated 1
Gene Ontology
HyperlinkGene Summary
This gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. [provided by RefSeq
Other Designations
OTTHUMP00000020121|epilepsy, partial
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Interactome
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