DCAMKL1 monoclonal antibody (M03), clone 6H4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DCAMKL1.
Immunogen
DCAMKL1 (NP_004725, 640 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QVLEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGVSVIALDHGFTIKRSGSLDYYQQPGMYWIRPPLLIRRGRFSDEDATRM
Host
Mouse
Reactivity
Human, Mouse
Interspecies Antigen Sequence
Mouse (96)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
DCAMKL1 monoclonal antibody (M03), clone 6H4 Western Blot analysis of DCAMKL1 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DCLK1 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to DCAMKL1 on NIH/3T3 cell. [antibody concentration 10 ug/ml] -
Gene Info — DCLK1
Entrez GeneID
9201GeneBank Accession#
NM_004734Protein Accession#
NP_004725Gene Name
DCLK1
Gene Alias
DCAMKL1, DCDC3A, DCLK, KIAA0369
Gene Description
doublecortin-like kinase 1
Omim ID
604742Gene Ontology
HyperlinkGene Summary
DCAMKL1 is a microtubule-associated protein that phosphorylates itself and myelin basic protein (MBP; MIM 159430). DCAMKL1 has microtubule polymerizing activity that is independent of its protein kinase activity (Lin et al., 2000 [PubMed 11124993]).[supplied by OMIM
Other Designations
OTTHUMP00000018250|doublecortin and CaM kinase-like 1
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Interactome
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Disease
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Publication Reference
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Mouse Intestinal Tuft Cells Express Advillin but Not Villin.
Amin Esmaeilniakooshkghazi, Sudeep P George, Ritwika Biswas, Seema Khurana.
Scientific Reports 2020 Jun; 10(1):8877.
Application:IF, IHC-P, Mouse, Mouse distal ileum.
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Mouse Intestinal Tuft Cells Express Advillin but Not Villin.
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