ZW10 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ZW10 transfected lysate.
Western Blot
Lane 1: ZW10 transfected lysate ( 88.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ZW10 full-length
Host
Human
Theoretical MW (kDa)
88.8
Interspecies Antigen Sequence
Mouse (86); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ZW10 antibody (H00009183-D01P) by Western Blots.
SDS-PAGE Gel
ZW10 transfected lysate.
Western Blot
Lane 1: ZW10 transfected lysate ( 88.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ZW10
Entrez GeneID
9183GeneBank Accession#
NM_004724Protein Accession#
NP_004715.1Gene Name
ZW10
Gene Alias
HZW10, KNTC1AP, MGC149821
Gene Description
ZW10, kinetochore associated, homolog (Drosophila)
Omim ID
603954Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase. [provided by RefSeq
Other Designations
ZW10 homolog, centromere/kinetochore protein|centromere/kinetochore protein zw10|zeste white 10 homolog
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