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Last updated: 2017/7/23
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ZW10 monoclonal antibody (M05), clone 2E5

  • Catalog # : H00009183-M05
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant ZW10.
  • Immunogen:
  • ZW10 (NP_004715, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • masfvtevla hsgrlekedl gtrisrltrr veeikgevcn miskkysefl psmqsaqgli tqvdklsedi dllksriese vrrdlhvstg eftdlkqqle
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00009183-M05
    Western Blot detection against Immunogen (36.63 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5.

    Lane 1: ZW10 transfected lysate(88.8 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi ( Cat # H00009183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZW10 monoclonal antibody (M05), clone 2E5 (Cat # H00009183-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9183
  • Gene Name:
  • ZW10
  • Gene Alias:
  • HZW10,KNTC1AP,MGC149821
  • Gene Description:
  • ZW10, kinetochore associated, homolog (Drosophila)
  • Gene Summary:
  • This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase. [provided by RefSeq
  • Other Designations:
  • ZW10 homolog, centromere/kinetochore protein,centromere/kinetochore protein zw10,zeste white 10 homolog
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