CTDP1 monoclonal antibody (M04), clone 3G4
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant CTDP1.
Immunogen
CTDP1 (AAH63447.1, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PPGSRESQTRKKVNHSRGTEVSEPSPPVRDPEGVTQAPGVEPSNGLEKPARELNGSEAATPRDSPRPGKPDERDIWPPAQAPTSSQELAGAPEPQGSCAQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (47); Rat (52)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CTDP1 monoclonal antibody (M04), clone 3G4. Western Blot analysis of CTDP1 expression in HepG2(Cat # L019V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CTDP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CTDP1 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CTDP1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — CTDP1
Entrez GeneID
9150GeneBank Accession#
BC063447Protein Accession#
AAH63447.1Gene Name
CTDP1
Gene Alias
CCFDN, FCP1
Gene Description
CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex. Alternative splicing of this gene results in two transcript variants encoding 2 different isoforms. [provided by RefSeq
Other Designations
CTD of POLR2A, phosphatase of, subunit 1|TFIIF-associating CTD phosphatase 1|serine phosphatase FCP1a|transcription factor IIF-associating CTD phosphatase 1
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Interactomes
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Diseases
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Publication Reference
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Active transcription and essential role of RNA polymerase II at the centromere during mitosis.
Chan FL, Marshall OJ, Saffery R, Kim BW, Earle E, Choo KH, Wong LH.
PNAS 2012 Feb; 109(6):1979.
Application:IF, Human, Mouse, HeLa, NIH/3T3 cells.
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Active transcription and essential role of RNA polymerase II at the centromere during mitosis.
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