ATG12 monoclonal antibody (M03), clone 1F9
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ATG12.
Immunogen
ATG12 (AAH12266, 1 a.a. ~ 140 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (85)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (41.14 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — ATG12
Entrez GeneID
9140GeneBank Accession#
BC012266Protein Accession#
AAH12266Gene Name
ATG12
Gene Alias
APG12, APG12L, FBR93, HAPG12
Gene Description
ATG12 autophagy related 12 homolog (S. cerevisiae)
Omim ID
609608Gene Ontology
HyperlinkGene Summary
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM
Other Designations
APG12 autophagy 12-like|Apg12 (autophagy, yeast) homolog
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Interactome
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Pathway
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Disease
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