ARHGEF1 monoclonal antibody (M02), clone 2D2
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ARHGEF1.
Immunogen
ARHGEF1 (AAH34013.2, 830 a.a. ~ 927 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
CRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (89)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.41 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ARHGEF1 expression in transfected 293T cell line by ARHGEF1 monoclonal antibody (M02), clone 2D2.
Lane 1: ARHGEF1 transfected lysate (Predicted MW: 102.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARHGEF1 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — ARHGEF1
Entrez GeneID
9138GeneBank Accession#
BC034013Protein Accession#
AAH34013.2Gene Name
ARHGEF1
Gene Alias
GEF1, LBCL2, LSC, P115-RHOGEF, SUB1.5
Gene Description
Rho guanine nucleotide exchange factor (GEF) 1
Omim ID
601855Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
115-kD protein|Lsc homolog|Rho guanine nucleotide exchange factor 1
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Interactome
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Pathway
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Disease
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