PDCD8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: AIFM1 transfected lysate ( 66.9 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
AIFM1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PDCD8 full-length
Host
Human
Theoretical MW (kDa)
67.54
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PDCD8 antibody (H00009131-B01) by Western Blots.
Western Blot
Lane 1: AIFM1 transfected lysate ( 66.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
AIFM1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AIFM1
Entrez GeneID
9131GeneBank Accession#
NM_004208Protein Accession#
NP_004199Gene Name
AIFM1
Gene Alias
AIF, MGC111425, PDCD8
Gene Description
apoptosis-inducing factor, mitochondrion-associated, 1
Omim ID
300169Gene Ontology
HyperlinkGene Summary
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Several alternative transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000024007|OTTHUMP00000024008|OTTHUMP00000024009|programmed cell death 8|programmed cell death 8 (apoptosis-inducing factor)|striatal apoptosis-inducing factor
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Interactome
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Pathway
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