PRPF4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PRPF4 transfected lysate.
Western Blot
Lane 1: PRPF4 transfected lysate ( 58.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRPF4 full-length
Host
Human
Theoretical MW (kDa)
58.4
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRPF4 antibody (H00009128-B01) by Western Blots.
SDS-PAGE Gel
PRPF4 transfected lysate.
Western Blot
Lane 1: PRPF4 transfected lysate ( 58.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRPF4
Entrez GeneID
9128GeneBank Accession#
NM_004697Protein Accession#
NP_004688Gene Name
PRPF4
Gene Alias
HPRP4, HPRP4P, PRP4, Prp4p
Gene Description
PRP4 pre-mRNA processing factor 4 homolog (yeast)
Omim ID
607795Gene Ontology
HyperlinkGene Summary
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. PRPF4 is 1 of several proteins that associate with U4 and U6 snRNPs.[supplied by OMIM
Other Designations
OTTHUMP00000021947|PRP4 pre-mRNA processing factor 4 homolog|PRP4/STK/WD splicing factor|U4/U6 small nuclear ribonucleoprotein Prp4
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