SMC3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CSPG6 transfected lysate.
Western Blot
Lane 1: CSPG6 transfected lysate ( 141.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SMC3 full-length
Host
Human
Theoretical MW (kDa)
141.5
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SMC3 antibody (H00009126-B01) by Western Blots.
SDS-PAGE Gel
CSPG6 transfected lysate.
Western Blot
Lane 1: CSPG6 transfected lysate ( 141.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SMC3
Entrez GeneID
9126GeneBank Accession#
BC047324Protein Accession#
AAH47324Gene Name
SMC3
Gene Alias
BAM, BMH, CDLS3, CSPG6, HCAP, SMC3L1
Gene Description
structural maintenance of chromosomes 3
Gene Ontology
HyperlinkGene Summary
This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq
Other Designations
OTTHUMP00000020477|bamacan proteoglycan|basement membrane-associated chondroitin proteoglycan|chondroitin sulfate proteoglycan 6 (bamacan)|chromosome-associated polypeptide
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Interactome
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Pathway
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Disease
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