LATS1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
LATS1 transfected lysate.
Western Blot
Lane 1: LATS1 transfected lysate ( 126.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-LATS1 full-length
Host
Human
Theoretical MW (kDa)
126.9
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LATS1 antibody (H00009113-B01) by Western Blots.
SDS-PAGE Gel
LATS1 transfected lysate.
Western Blot
Lane 1: LATS1 transfected lysate ( 126.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — LATS1
Entrez GeneID
9113GeneBank Accession#
NM_004690.2Protein Accession#
NP_004681.1Gene Name
LATS1
Gene Alias
WARTS, wts
Gene Description
LATS, large tumor suppressor, homolog 1 (Drosophila)
Omim ID
603473Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq
Other Designations
LATS (large tumor suppressor, Drosophila) homolog 1|LATS homolog 1
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Interactome
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Disease
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