RGN 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
Western Blot
Lane 1: RGN transfected lysate ( 33.3 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
RGN transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RGN full-length
Host
Human
Theoretical MW (kDa)
33
Interspecies Antigen Sequence
Rat (88)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RGN antibody (H00009104-B01) by Western Blots.
Western Blot
Lane 1: RGN transfected lysate ( 33.3 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
RGN transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RGN
Entrez GeneID
9104GeneBank Accession#
NM_004683.4Protein Accession#
-Gene Name
RGN
Gene Alias
RC, SMP30
Gene Description
regucalcin (senescence marker protein-30)
Omim ID
300212Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in two transcript variants having different 5' UTRs, but encoding the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000023195|OTTHUMP00000023196|regucalcin|senescence marker protein-30
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