USP14 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-USP14 full-length
Host
Human
Theoretical MW (kDa)
56.1
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-USP14 antibody (H00009097-D01P) by Western Blots.
SDS-PAGE Gel
USP14 transfected lysate.
Western Blot
Lane 1: USP14 transfected lysate ( 56.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — USP14
Entrez GeneID
9097GeneBank Accession#
NM_005151.3Protein Accession#
NP_005142.1Gene Name
USP14
Gene Alias
TGT
Gene Description
ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Omim ID
607274Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
deubiquitinating enzyme 14|tRNA-guanine transglycosylase, 60-kD subunit|ubiquitin carboxyl-terminal hydrolase 14|ubiquitin specific protease 14|ubiquitin specific protease 14 (tRNA-guanine transglycosylase)|ubiquitin thiolesterase 14|ubiquitin-specific pr
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