This antibody pair set comes with matched antibody pair to detect and quantify protein level of human USP14.
Quality Control Testing:
Standard curve using USP14 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 243x to 3x dilution of the USP14 293T overexpression lysate (non-denatured).
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-USP14 (100 ug) 2. Detection antibody: rabbit purified polyclonal anti-USP14 (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
deubiquitinating enzyme 14,tRNA-guanine transglycosylase, 60-kD subunit,ubiquitin carboxyl-terminal hydrolase 14,ubiquitin specific protease 14,ubiquitin specific protease 14 (tRNA-guanine transglycosylase),ubiquitin thiolesterase 14,ubiquitin-specific pr