PAPSS1 monoclonal antibody (M05), clone 1F4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PAPSS1 expression in transfected 293T cell line by PAPSS1 monoclonal antibody (M05), clone 1F4.
Lane 1: PAPSS1 transfected lysate(68.5 KDa).
Lane 2: Non-transfected lysate.
RNAi Knockdown (Antibody validated)
Western blot analysis of PAPSS1 over-expressed 293 cell line, cotransfected with PAPSS1 Validated Chimera RNAi ( Cat # H00009061-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PAPSS1 monoclonal antibody (M05), clone 1F4 (Cat # H00009061-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PAPSS1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PAPSS1.
Immunogen
PAPSS1 (NP_005434, 144 a.a. ~ 252 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (90)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PAPSS1 expression in transfected 293T cell line by PAPSS1 monoclonal antibody (M05), clone 1F4.
Lane 1: PAPSS1 transfected lysate(68.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PAPSS1 over-expressed 293 cell line, cotransfected with PAPSS1 Validated Chimera RNAi ( Cat # H00009061-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PAPSS1 monoclonal antibody (M05), clone 1F4 (Cat # H00009061-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to PAPSS1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PAPSS1
Entrez GeneID
9061GeneBank Accession#
NM_005443Protein Accession#
NP_005434Gene Name
PAPSS1
Gene Alias
ATPSK1, PAPSS, SK1
Gene Description
3'-phosphoadenosine 5'-phosphosulfate synthase 1
Omim ID
603262Gene Ontology
HyperlinkGene Summary
Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM
Other Designations
3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1
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Interactome
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Pathway
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Disease
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