NFS1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NFS1 full-length
Host
Human
Theoretical MW (kDa)
50.38
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NFS1 antibody (H00009054-B01) by Western Blots.
SDS-PAGE Gel
NFS1 transfected lysate.
Western Blot
Lane 1: NFS1 transfected lysate ( 50.38 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NFS1
Entrez GeneID
9054GeneBank Accession#
NM_021100.3Protein Accession#
NP_066923.3Gene Name
NFS1
Gene Alias
HUSSY-08, IscS, NIFS
Gene Description
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Omim ID
603485Gene Ontology
HyperlinkGene Summary
Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. [provided by RefSeq
Other Designations
NFS1 nitrogen fixation 1|OTTHUMP00000030799|cysteine desulfurase|nitrogen fixation 1 (S. cerevisiae, homolog)|nitrogen-fixing bacteria S-like protein
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Interactome
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Pathway
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