NFS1 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00009054-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

NFS1 transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: NFS1 transfected lysate ( 50.38 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-NFS1 full-length

    Host

    Human

    Theoretical MW (kDa)

    50.38

    Interspecies Antigen Sequence

    Mouse (91); Rat (91)

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-NFS1 antibody (H00009054-B01) by Western Blots.

    SDS-PAGE Gel

    NFS1 transfected lysate.

    Western Blot

    Lane 1: NFS1 transfected lysate ( 50.38 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — NFS1

    Entrez GeneID

    9054

    GeneBank Accession#

    NM_021100.3

    Protein Accession#

    NP_066923.3

    Gene Name

    NFS1

    Gene Alias

    HUSSY-08, IscS, NIFS

    Gene Description

    NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

    Omim ID

    603485

    Gene Ontology

    Hyperlink

    Gene Summary

    Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. [provided by RefSeq

    Other Designations

    NFS1 nitrogen fixation 1|OTTHUMP00000030799|cysteine desulfurase|nitrogen fixation 1 (S. cerevisiae, homolog)|nitrogen-fixing bacteria S-like protein

  • Interactome
  • Pathway
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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