NFS1 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of NFS1 expression in transfected 293T cell line (H00009054-T01) by NFS1 MaxPab polyclonal antibody.
Lane1:NFS1 transfected lysate(50.27 KDa).
Lane2:Non-transfected lysate.
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human NFS1 protein.
Immunogen
NFS1 (NP_066923.3, 1 a.a. ~ 457 a.a) full-length human protein.
Sequence
MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of NFS1 expression in transfected 293T cell line (H00009054-T01) by NFS1 MaxPab polyclonal antibody.
Lane1:NFS1 transfected lysate(50.27 KDa).
Lane2:Non-transfected lysate.
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Gene Info — NFS1
Entrez GeneID
9054GeneBank Accession#
NM_021100.3Protein Accession#
NP_066923.3Gene Name
NFS1
Gene Alias
HUSSY-08, IscS, NIFS
Gene Description
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Omim ID
603485Gene Ontology
HyperlinkGene Summary
Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. [provided by RefSeq
Other Designations
NFS1 nitrogen fixation 1|OTTHUMP00000030799|cysteine desulfurase|nitrogen fixation 1 (S. cerevisiae, homolog)|nitrogen-fixing bacteria S-like protein
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Interactome
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Pathway
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Publication Reference
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Elucidation of the mechanism of mitochondrial iron loading in Friedreich's ataxia by analysis of a mouse mutant.
Huang ML, Becker EM, Whitnall M, Rahmanto YS, Ponka P, Richardson DR.
PNAS 2009 Sep; 106(38):16381.
Application：WB-Ti, Mouse, Mouse hearts.
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Elucidation of the mechanism of mitochondrial iron loading in Friedreich's ataxia by analysis of a mouse mutant.
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