RNF8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RNF8 transfected lysate.
Western Blot
Lane 1: RNF8 transfected lysate ( 53.46 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RNF8 full-length
Host
Human
Theoretical MW (kDa)
53.46
Interspecies Antigen Sequence
Mouse (71); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RNF8 antibody (H00009025-B01) by Western Blots.
SDS-PAGE Gel
RNF8 transfected lysate.
Western Blot
Lane 1: RNF8 transfected lysate ( 53.46 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RNF8
Entrez GeneID
9025GeneBank Accession#
NM_003958.2Protein Accession#
NP_003949.1Gene Name
RNF8
Gene Alias
FLJ12013, KIAA0646
Gene Description
ring finger protein 8
Omim ID
611685Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
C3HC4-type zinc finger protein|OTTHUMP00000016323|OTTHUMP00000039684|UBC13/UEV-interacting ring finger protein|ring finger protein (C3HC4 type) 8
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Interactome
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Disease
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