TAF1A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TAF1A transfected lysate.
Western Blot
Lane 1: TAF1A transfected lysate ( 52.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TAF1A full-length
Host
Human
Theoretical MW (kDa)
52.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TAF1A antibody (H00009015-D01P) by Western Blots.
SDS-PAGE Gel
TAF1A transfected lysate.
Western Blot
Lane 1: TAF1A transfected lysate ( 52.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TAF1A
Entrez GeneID
9015GeneBank Accession#
NM_005681.2Protein Accession#
NP_005672.1Gene Name
TAF1A
Gene Alias
MGC:17061, RAFI48, SL1, TAFI48
Gene Description
TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
Omim ID
604903Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000035653|OTTHUMP00000035654|SL1, 48kD subunit|TBP-associated factor 1A
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