TAF1B MaxPab rabbit polyclonal antibody (D01)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of TAF1B expression in transfected 293T cell line (H00009014-T01) by TAF1B MaxPab polyclonal antibody.
Lane 1: TAF1B transfected lysate(68.80 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of TAF1B transfected lysate using anti-TAF1B MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with TAF1B purified MaxPab mouse polyclonal antibody (B01P) (H00009014-B01P).
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human TAF1B protein.
Immunogen
TAF1B (AAH18137.1, 1 a.a. ~ 588 a.a) full-length human protein.
Sequence
MDLEESEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTIEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIAKMAKAVKYDVQAVAIIVVVLKLLFLLDDSFEWSLSNLAEKHNEKNKKDKPWFDFRKWYQIMKKAFDEKKQKWEEARAKYLWKSEKPLYYSFVDKPVAYKKREMVVNLQKQFSTLVDSTATAGKKSPSSFQFNWTEEDTDRTCFHGHSLQGVLKEKGQSLLTKNSLYWLSTQKFCRCYCTHVTTYEESNYSLSYQFILNLFSFLLRIKTSLLHEEVSLVEKKLFEKKYSVKRKKSRSKKVRRH
Host
Rabbit
Reactivity
Human
Interspecies Antigen Sequence
Mouse (74); Rat (74)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TAF1B expression in transfected 293T cell line (H00009014-T01) by TAF1B MaxPab polyclonal antibody.
Lane 1: TAF1B transfected lysate(68.80 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of TAF1B transfected lysate using anti-TAF1B MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with TAF1B purified MaxPab mouse polyclonal antibody (B01P) (H00009014-B01P). -
Gene Info — TAF1B
Entrez GeneID
9014GeneBank Accession#
BC018137.2Protein Accession#
AAH18137.1Gene Name
TAF1B
Gene Alias
MGC:9349, RAF1B, RAFI63, SL1, TAFI63
Gene Description
TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
Omim ID
604904Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes one of the SL1-specific TAFs. [provided by RefSeq
Other Designations
SL1, 63kD subunit|TBP-associated factor 1B
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Interactome
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Disease
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Publication Reference
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The chemotherapeutic agent CX-5461 irreversibly blocks RNA polymerase I initiation and promoter release to cause nucleolar disruption, DNA damage and cell inviability.
Jean-Clément Mars, Michel G Tremblay, Mélissa Valere, Dany S Sibai, Marianne Sabourin-Felix, Frédéric Lessard, Tom Moss.
NAR Cancer 2020 Dec; 2(4):zcaa032.
Application：ChIP, Human, HEK 293T cells.
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The chemotherapeutic agent CX-5461 irreversibly blocks RNA polymerase I initiation and promoter release to cause nucleolar disruption, DNA damage and cell inviability.
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