PLOD3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PLOD3 transfected lysate.
Western Blot
Lane 1: PLOD3 transfected lysate ( 81.29 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PLOD3 full-length
Host
Human
Theoretical MW (kDa)
81.29
Interspecies Antigen Sequence
Mouse (91); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PLOD3 antibody (H00008985-B01) by Western Blots.
SDS-PAGE Gel
PLOD3 transfected lysate.
Western Blot
Lane 1: PLOD3 transfected lysate ( 81.29 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PLOD3
Entrez GeneID
8985GeneBank Accession#
NM_001084.4Protein Accession#
NP_001075.1Gene Name
PLOD3
Gene Alias
LH3
Gene Description
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Omim ID
603066Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq
Other Designations
lysine hydroxylase 3|lysyl hydroxylase 3
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Interactome
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Pathway
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Disease
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