PLOD3 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
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Western Blot (Cell lysate)
PLOD3 MaxPab polyclonal antibody. Western Blot analysis of PLOD3 expression in HepG2.
Western Blot (Transfected lysate)
Western Blot analysis of PLOD3 expression in transfected 293T cell line (H00008985-T01) by PLOD3 MaxPab polyclonal antibody.
Lane 1: PLOD3 transfected lysate(81.18 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to PLOD3 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human PLOD3 protein.
Immunogen
PLOD3 (NP_001075.1, 1 a.a. ~ 738 a.a) full-length human protein.
Sequence
MTSSGPGPRFLLLLPLLLPPAASASDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEEWRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTELLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (90)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PLOD3 MaxPab polyclonal antibody. Western Blot analysis of PLOD3 expression in HepG2.Western Blot (Transfected lysate)
Western Blot analysis of PLOD3 expression in transfected 293T cell line (H00008985-T01) by PLOD3 MaxPab polyclonal antibody.
Lane 1: PLOD3 transfected lysate(81.18 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to PLOD3 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — PLOD3
Entrez GeneID
8985GeneBank Accession#
NM_001084.4Protein Accession#
NP_001075.1Gene Name
PLOD3
Gene Alias
LH3
Gene Description
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Omim ID
603066Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq
Other Designations
lysine hydroxylase 3|lysyl hydroxylase 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach.
Amodio G, Sasso E, D'Ambrosio C, Scaloni A, Moltedo O, Franceschelli S, Zambrano N, Remondelli P.
Cell Biology and Toxicology 2016 Aug; 32(4):285.
Application：IF, WB-Tr, Human, Huh7, HeLa cells.
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Proteomic Signatures in Thapsigargin-Treated Hepatoma Cells.
Amodio G, Moltedo O, Monteleone F, D'Ambrosio C, Scaloni A, Remondelli P, Zambrano N.
Chem Res Toxicol 2011 Jul; 24:1215.
Application：WB-Ce, Human, Huh7 cells.
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Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach.
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