WASL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
WASL transfected lysate.
Western Blot
Lane 1: WASL transfected lysate ( 55.66 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WASL full-length
Host
Human
Theoretical MW (kDa)
55.66
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WASL antibody (H00008976-B01) by Western Blots.
SDS-PAGE Gel
WASL transfected lysate.
Western Blot
Lane 1: WASL transfected lysate ( 55.66 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WASL
Entrez GeneID
8976GeneBank Accession#
NM_003941.2Protein Accession#
NP_003932.3Gene Name
WASL
Gene Alias
DKFZp779G0847, MGC48327, N-WASP, NWASP
Gene Description
Wiskott-Aldrich syndrome-like
Omim ID
605056Gene Ontology
HyperlinkGene Summary
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq
Other Designations
Wiskott-Aldrich syndrome gene-like|Wiskott-Aldrich syndrome gene-like protein|neural Wiskott-Aldrich syndrome protein
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Interactome
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Pathway
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