SNURF 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SNURF full-length
Host
Human
Theoretical MW (kDa)
26.51
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SNURF antibody (H00008926-B01) by Western Blots.
SDS-PAGE Gel
SNURF transfected lysate.
Western Blot
Lane 1: SNURF transfected lysate ( 26.51 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SNURF
Entrez GeneID
8926GeneBank Accession#
BC024777Protein Accession#
AAH24777Gene Name
SNURF
Gene Alias
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Gene Description
SNRPN upstream reading frame
Gene Ontology
HyperlinkGene Summary
This gene encodes a highly basic protein localized to the nucleus. The evolutionarily constrained open reading frame is found on a bicistronic transcript which has a downstream ORF encoding the small nuclear ribonucleoprotein polypeptide N. The upstream coding region utilizes the first three exons of the transcript, a region that has been identified as an imprinting center. Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region but the full-length nature of these transcripts has not been determined. An alternate exon has been identified that substitutes for exon 4 and leads to a truncated, monocistronic transcript. Alternative splicing or deletion caused by a translocation event in the 5' UTR or coding region of this gene leads to Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. The function of this protein is not yet known. [provided by RefSeq
Other Designations
SNRPN upstream reading frame protein
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