MTMR3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MTMR3 transfected lysate.
Western Blot
Lane 1: MTMR3 transfected lysate ( 131.89 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MTMR3 full-length
Host
Human
Theoretical MW (kDa)
131.89
Interspecies Antigen Sequence
Mouse (84); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MTMR3 antibody (H00008897-B01) by Western Blots.
SDS-PAGE Gel
MTMR3 transfected lysate.
Western Blot
Lane 1: MTMR3 transfected lysate ( 131.89 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MTMR3
Entrez GeneID
8897GeneBank Accession#
NM_021090.2Protein Accession#
NP_066576.1Gene Name
MTMR3
Gene Alias
FLJ32333, FYVE-DSP1, KIAA0371, ZFYVE10
Gene Description
myotubularin related protein 3
Omim ID
603558Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the myotubularin dual specificity protein phosphatase gene family. The encoded protein is structurally similar to myotubularin but in addition contains a FYVE domain and an N-terminal PH-GRAM domain. The protein can self-associate and also form heteromers with another myotubularin related protein. The protein binds to phosphoinositide lipids through the PH-GRAM domain, and can hydrolyze phosphatidylinositol(3)-phosphate and phosphatidylinositol(3,5)-biphosphate in vitro. The encoded protein has been observed to have a perinuclear, possibly membrane-bound, distribution in cells, but it has also been found free in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
FYVE (Fab1 YGLO23 Vsp27 EEA1 domain) dual-specificity protein phosphatase|myotubularin-related protein 3|zinc finger, FYVE domain containing 10
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Interactome
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