EIF2B2 monoclonal antibody (M01), clone 5B12-E10
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More Files
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Western Blot (Cell lysate)
EIF2B2 monoclonal antibody (M01), clone 5B12-E10 Western Blot analysis of EIF2B2 expression in K-562 ( Cat # L009V1 ).
Western Blot (Cell lysate)
EIF2B2 monoclonal antibody (M01), clone 5B12-E10. Western Blot analysis of EIF2B2 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of EIF2B2 expression in transfected 293T cell line by EIF2B2 monoclonal antibody (M01), clone 5B12-E10.
Lane 1: EIF2B2 transfected lysate(39 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to EIF2B2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 5 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EIF2B2 is approximately 0.3ng/ml as a capture antibody.
Western Blot detection against Immunogen (64.35 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant EIF2B2.
Immunogen
EIF2B2 (AAH00494, 1 a.a. ~ 351 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRTDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (93)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (64.35 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
EIF2B2 monoclonal antibody (M01), clone 5B12-E10 Western Blot analysis of EIF2B2 expression in K-562 ( Cat # L009V1 ).Western Blot (Cell lysate)
EIF2B2 monoclonal antibody (M01), clone 5B12-E10. Western Blot analysis of EIF2B2 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of EIF2B2 expression in transfected 293T cell line by EIF2B2 monoclonal antibody (M01), clone 5B12-E10.
Lane 1: EIF2B2 transfected lysate(39 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to EIF2B2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EIF2B2 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — EIF2B2
Entrez GeneID
8892GeneBank Accession#
BC000494Protein Accession#
AAH00494Gene Name
EIF2B2
Gene Alias
EIF-2Bbeta, EIF2B
Gene Description
eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
Gene Ontology
HyperlinkGene Summary
Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon (EIF2B5; MIM 603945) subunits. EIF2B activates its EIF2 (see MIM 603907) substrate by exchanging EIF2-bound GDP for GTP.[supplied by OMIM
Other Designations
eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD)
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Interactome
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Disease
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