APPBP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
APPBP1 transfected lysate
Western Blot
Lane 1: APPBP1 transfected lysate ( 60.2 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-APPBP1 full-length
Host
Human
Theoretical MW (kDa)
60.2
Interspecies Antigen Sequence
Mouse (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-APPBP1 antibody (H00008883-B01) by Western Blots.
SDS-PAGE Gel
APPBP1 transfected lysate
Western Blot
Lane 1: APPBP1 transfected lysate ( 60.2 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NAE1
Entrez GeneID
8883GeneBank Accession#
NM_003905Protein Accession#
NP_003896Gene Name
NAE1
Gene Alias
A-116A10.1, APPBP1, HPP1, ula-1
Gene Description
NEDD8 activating enzyme E1 subunit 1
Omim ID
603385Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
NEDD8-activating enzyme E1 subunit|amyloid beta precursor protein binding protein 1, 59kDa|amyloid beta precursor protein-binding protein 1, 59kD|amyloid protein-binding protein 1|protooncogene protein 1
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