SQSTM1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SQSTM1 transfected lysate.
Western Blot
Lane 1: SQSTM1 transfected lysate ( 47.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SQSTM1 full-length
Host
Human
Theoretical MW (kDa)
47.7
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SQSTM1 antibody (H00008878-D01P) by Western Blots.
SDS-PAGE Gel
SQSTM1 transfected lysate.
Western Blot
Lane 1: SQSTM1 transfected lysate ( 47.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SQSTM1
Entrez GeneID
8878GeneBank Accession#
NM_003900Protein Accession#
NP_003891.1Gene Name
SQSTM1
Gene Alias
A170, OSIL, PDB3, ZIP3, p60, p62, p62B
Gene Description
sequestosome 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq
Other Designations
EBI3-associated protein p60|Paget disease of bone 3|oxidative stress induced like|phosphotyrosine independent ligand for the Lck SH2 domain p62|ubiquitin-binding protein p62
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Interactome
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Disease
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