SQSTM1 monoclonal antibody (M01J), clone 2C11
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More Files
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Western Blot (Cell lysate)
SQSTM1 monoclonal antibody (M01J), clone 2C11. Western Blot analysis of SQSTM1 expression in HeLa.
Western Blot (Cell lysate)
SQSTM1 monoclonal antibody (M01J), clone 2C11. Western Blot analysis of SQSTM1 expression in MCF-7.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SQSTM1 is 1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to SQSTM1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (74.14 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant SQSTM1.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
SQSTM1 (AAH03139.1, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Preparation Method
Cell Culture Production
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (74.14 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SQSTM1 monoclonal antibody (M01J), clone 2C11. Western Blot analysis of SQSTM1 expression in HeLa.Western Blot (Cell lysate)
SQSTM1 monoclonal antibody (M01J), clone 2C11. Western Blot analysis of SQSTM1 expression in MCF-7.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SQSTM1 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SQSTM1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — SQSTM1
Entrez GeneID
8878GeneBank Accession#
BC003139Protein Accession#
AAH03139.1Gene Name
SQSTM1
Gene Alias
A170, OSIL, PDB3, ZIP3, p60, p62, p62B
Gene Description
sequestosome 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq
Other Designations
EBI3-associated protein p60|Paget disease of bone 3|oxidative stress induced like|phosphotyrosine independent ligand for the Lck SH2 domain p62|ubiquitin-binding protein p62
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Interactome
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Disease
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Publication Reference
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Toxoplasma IWS1 Determines Fitness in Interferon-γ-Activated Host Cells and Mice by Indirectly Regulating ROP18 mRNA Expression.
Emi Hashizaki, Miwa Sasai, Daisuke Okuzaki, Tsubasa Nishi, Takashi Kobayashi, Shiroh Iwanaga, Masahiro Yamamoto.
Mbio 2023 Feb; 14(1):e0325622.
Application：IF, Mouse, MEFs.
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Toxoplasma IWS1 Determines Fitness in Interferon-γ-Activated Host Cells and Mice by Indirectly Regulating ROP18 mRNA Expression.
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