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SPHK1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00008877-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-SPHK1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 43.9
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-SPHK1 antibody (H00008877-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00008877-T02
    SPHK1 transfected lysate.
    Western Blot
    QC Testing of H00008877-T02
    Lane 1: SPHK1 transfected lysate ( 43.90 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 8877
  • Gene Name:
  • SPHK1
  • Gene Alias:
  • SPHK
  • Gene Description:
  • sphingosine kinase 1
  • Gene Summary:
  • Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM
  • Other Designations:
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