SPHK1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: SPHK1 transfected lysate ( 43.9 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
SPHK1 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SPHK1 full-length
Host
Human
Theoretical MW (kDa)
43.89
Interspecies Antigen Sequence
Mouse (81); Rat (78)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SPHK1 antibody (H00008877-B01) by Western Blots.
Western Blot
Lane 1: SPHK1 transfected lysate ( 43.9 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
SPHK1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SPHK1
Entrez GeneID
8877GeneBank Accession#
NM_021972Protein Accession#
NP_068807Gene Name
SPHK1
Gene Alias
SPHK
Gene Description
sphingosine kinase 1
Omim ID
603730Gene Ontology
HyperlinkGene Summary
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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