SPHK1 monoclonal antibody (M01), clone 1D6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SPHK1.
Immunogen
SPHK1 (AAH08040, 1 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (78)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.98 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SPHK1 monoclonal antibody (M01), clone 1D6. Western Blot analysis of SPHK1 expression in K-562.Western Blot (Transfected lysate)
Western Blot analysis of SPHK1 expression in transfected 293T cell line by SPHK1 monoclonal antibody (M01), clone 1D6.
Lane 1: SPHK1 transfected lysate(43.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPHK1 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — SPHK1
Entrez GeneID
8877GeneBank Accession#
BC008040Protein Accession#
AAH08040Gene Name
SPHK1
Gene Alias
SPHK
Gene Description
sphingosine kinase 1
Omim ID
603730Gene Ontology
HyperlinkGene Summary
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Publication Reference
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C16-ceramide and sphingosine 1-phosphate/S1PR2 have opposite effects on cell growth through mTOR signaling pathway regulation.
Kim MH, Park JW, Lee EJ, Kim S, Shin SH, Ahn JH, Jung Y, Park I, Park WJ.
Oncology Reports 2018 Nov; 40(5):2977.
Application:WB, Human, BT‑474, MCF‑7, MDA-MB-361 cells.
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MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1.
Zhang H, Wang Q, Zhao Q, Di W.
Journal of Ovarian Research 2013 Nov; 6(1):84.
Application:WB-Tr, Human, SKOV3-ip cells.
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Factor-Xa-induced mitogenesis and migration require sphingosine kinase activity and S1P formation in human vascular smooth muscle cells.
Bohm A, Flosser A, Ermler S, Fender AC, Luth A, Kleuser B, Schror K, Rauch BH.
Cardiovascular Research 2013 Aug; 99(3):505.
Application:IHC, IF, WB-Ce, Human, Smooth muscle cells, Carotid artery.
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LRIG1 modulates aggressiveness of head and neck cancers by regulating EGFR-MAPK-SPHK1 signaling and extracellular matrix remodeling.
Sheu JJ, Lee CC, Hua CH, Li CI, Lai MT, Lee SC, Cheng J, Chen CM, Chan C, Chao SC, Chen JY, Chang JY, Lee CH.
Oncogene 2014 Mar; 33(11):1375.
Application:WB-Ce, Human, TW01-LG1/a cells.
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The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen.
Schroder M, Richter C, Juan MH, Maltusch K, Giegold O, Quintini G, Pfeilschifter JM, Huwiler A, Radeke HH.
Mol Immunol 2011 Mar; 48:1139.
Application:WB-Ce, Mouse, Splenocytes.
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The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line.
Asher V, Warren A, Shaw R, Sowter H, Bali A, Khan R.
Cancer Cell Int 2011 Mar; 11:6.
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Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition.
Sinha UK, Schorn VJ, Hochstim C, Chinn SB, Zhu S, Masood R.
Head & Neck 2011 Feb; 33(2):178.
Application:IHC-P, WB-Ce, WB-Tr, Human, Head and neck squamous cell carcinoma tissues, SCC-15, SCC-25 cells.
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Effect of HFE Variants on Sphingolipid Expression by SH-SY5Y Human Neuroblastoma Cells.
Ali-Rahmani F, Hengst JA, Connor JR, Schengrund CL.
Neurochemical Research 2011 Sep; 36(9):1687.
Application:WB-Ce, Human, SH-SY5Y cells.
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Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9.
Schenten V, Melchior C, Steinckwich N, Tschirhart EJ, Brechard S.
J Leukoc Biol 2011 Jan; 89:587.
Application:WB-Tr, Human, dHL-60 cells.
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A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart.
Wang S, Zhang Z, Lin X, Xu DS, Feng Y, Ding K.
Glycobiology 2010 Jan; 20(4):473.
Application:WB, Human, Human microvascular endothelial cells.
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G{alpha}q-mediated plasma membrane translocation of sphingosine kinase-1 and cross-activation of S1P receptors.
Ter Braak M, Danneberg K, Lichte K, Liphardt K, Ktistakis NT, Pitson SM, Hla T, Jakobs KH, Heringdorf DM.
Biochimica et Biophysica Acta 2009 May; 1791(5):357.
Application:WB-Ce, Human, HEK 293 cells.
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Sphingosine kinase 1 is essential for proteinase-activated receptor-1 signalling in epithelial and endothelial cells.
Billich A, Urtz N, Reuschel R, Baumruker T.
The International Journal of Biochemistry & Cell Biology 2009 Jul; 41(7):1547.
Application:WB-Tr, Human, A-549 cells.
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C16-ceramide and sphingosine 1-phosphate/S1PR2 have opposite effects on cell growth through mTOR signaling pathway regulation.
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