SPHK1 (Human) Matched Antibody Pair
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More Files
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Sandwich ELISA detection sensitivity ranging from approximately 729x to 3x dilution of the SPHK1 293T overexpression lysate (non-denatured).
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Specification
Product Description
This antibody pair set comes with a matched antibody pair to detect and quantify the protein level of human SPHK1.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (78)
Quality Control Testing
Standard curve using SPHK1 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 729x to 3x dilution of the SPHK1 293T overexpression lysate (non-denatured).
Supplied Product
Antibody pair set content:
1. Capture antibody: mouse monoclonal anti-SPHK1 (100 ug)
2. Detection antibody: rabbit purified polyclonal anti-SPHK1 (50 ug)
*Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
ELISA Pair (Transfected lysate)
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Gene Info — SPHK1
Entrez GeneID
8877Gene Name
SPHK1
Gene Alias
SPHK
Gene Description
sphingosine kinase 1
Omim ID
603730Gene Ontology
HyperlinkGene Summary
Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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