VNN1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
VNN1 transfected lysate.
Western Blot
Lane 1: VNN1 transfected lysate ( 56.54 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VNN1 full-length
Host
Human
Theoretical MW (kDa)
56.54
Interspecies Antigen Sequence
Mouse (75); Rat (78)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VNN1 antibody (H00008876-B01) by Western Blots.
SDS-PAGE Gel
VNN1 transfected lysate.
Western Blot
Lane 1: VNN1 transfected lysate ( 56.54 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VNN1
Entrez GeneID
8876GeneBank Accession#
NM_004666.1Protein Accession#
NP_004657.1Gene Name
VNN1
Gene Alias
HDLCQ8, MGC116930, MGC116931, MGC116932, MGC116933, Tiff66
Gene Description
vanin 1
Omim ID
603570Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. [provided by RefSeq
Other Designations
OTTHUMP00000017227|Vannin 1|pantetheinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Vanin-1 pantetheinase drives smooth muscle cell activation in post-arterial injury neointimal hyperplasia.
Dammanahalli KJ, Stevens S, Terkeltaub R.
PLoS One 2012 Jun; 7(6):e39106.
Application：Func, Mouse, Mouse arterial sections and SMC lysates.
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Vanin-1 pantetheinase drives smooth muscle cell activation in post-arterial injury neointimal hyperplasia.
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