ARHGEF7 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ARHGEF7 transfected lysate.
Western Blot
Lane 1: ARHGEF7 transfected lysate ( 73.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ARHGEF7 full-length
Host
Human
Theoretical MW (kDa)
73.1
Interspecies Antigen Sequence
Mouse (93); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ARHGEF7 antibody (H00008874-D01P) by Western Blots.
SDS-PAGE Gel
ARHGEF7 transfected lysate.
Western Blot
Lane 1: ARHGEF7 transfected lysate ( 73.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ARHGEF7
Entrez GeneID
8874GeneBank Accession#
NM_003899.2Protein Accession#
NP_003890.1Gene Name
ARHGEF7
Gene Alias
BETA-PIX, COOL1, DKFZp686C12170, DKFZp761K1021, KIAA0142, KIAA0412, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB
Gene Description
Rho guanine nucleotide exchange factor (GEF) 7
Omim ID
605477Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes triggered by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. This protein can induce membrane ruffling. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000178804|PAK-interacting exchange factor beta|SH3 domain-containing proline-rich protein|guanine nucleotide exchange factor 7|rho guanine nucleotide exchange factor 7
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Interactome
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Pathway
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