ALKBH 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ALKBH full-length
Host
Human
Theoretical MW (kDa)
42.9
Interspecies Antigen Sequence
Mouse (82); Rat (75)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALKBH antibody (H00008846-B01) by Western Blots.
Western Blot
Lane 1: ALKBH1 transfected lysate ( 42.79 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
ALKBH1 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ALKBH1
Entrez GeneID
8846GeneBank Accession#
BC025787Protein Accession#
AAH25787Gene Name
ALKBH1
Gene Alias
ABH, ABH1, ALKBH, alkB, hABH
Gene Description
alkB, alkylation repair homolog 1 (E. coli)
Omim ID
605345Gene Ontology
HyperlinkGene Summary
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq
Other Designations
alkylated DNA repair protein alkB homolog|alkylation repair, alkB homolog
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